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Determination of Pre Harvest Interval for Fenitrothion and Cypermethrin in Major Vegetables

M. S. Ahmed
Senior Scientific Officer
Entomology Division, BARI, Gazipur

M. A. Rahman
Senior Scientific Officer
Entomology Division, BARI, Gazipur

M. W. Akon
Senior Scientific Officer
Entomology Division, BARI, Gazipur

Afroza Begum
Scientific Officer
Entomology Division, BARI, Gazipur

Abstract/ Executive Summary:

A study was carried out to determine the per harvest interval (PHI) for fenitrothion in brinjal, country bean, yard long bean, cabbage, cauliflower, tomato, chilli, stem amaranth and red amaranth; cypermethrin in yard long bean, chilli, stem amaranth and red amaranth depending on Maximum Residue Limit (MRL) Set by FAO/WHO.. The quantities were above MRL up to 8 DAS for fenitrothion in cauliflower; 7 DAS for fenitrothion in bean, brinjal, stem amaranth and red amaranth; 6 DAS for fenitrothion in cabbage and chilli; 5 DAS for fenitrothion in tomato; 4 DAS for cypermethrin in red amaranth and 3 DAS for cypermethrin in yard long bean, chilli and stem amaranth. At 10 DAS, no residue was detected from any of the tested samples except fenitrothion in cauliflower and cypermethrin in yard long bean. The determined PHI were 9 DAS for fenitrothion in cauliflower; 8 DAS for fenitrothion in bean, brinjal, stem amaranth and red amaranth; 7 DAS for fenitrothion in cabbage and chilli; 6 DAS for fenitrothion in tomato; 5 DAS for cypermethrin in red amaranth and 4 DAS for cypermethrin in yard long bean, chilli and stem amaranth.

Keywords: Pre Harvest Interval , Fenitrothion, Cypermethrin, Vegetables

Location: Gazipur

Start Date: 10-10-2012

End Date: 05-05-2013

Program Area: Pest Management

Commodity:

Objectives:

1. To determine the pre harvest intervals (PHI) for Fenitrothion, Quinalphos and Cypermethrin in bean, yard long bean, brinjal, cabbage, cauliflower, tomato, chilli, stem amaranth and red amaranth; 2.To set a standard parameter for Good Agricultural Practice (GAP).

Methodology:

The standard for fenitrothion and cypermethrin contained 99.6% purity. Marketable size of brinjal, country bean, yard long bean, cabbage, cauliflower, tomato, chilli, stem amaranth and red amaranth were collected from supervised field trials at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11 days after spray (DAS) which were sprayed with fenitrothion @ 2 ml/L of water and cypermethrin @ 1 ml/L of water. Extraction and separation: Field collected samples (250g) were grounded thoroughly with the meat grinder. A sub sample of 20g was taken into a wide mouth jar then 100 ml of hexane was added to it. Sodium sulphate (Na2SO4) was also added with sample until water was removed from the sample. The mixture was then macerated with high-speed homogenizer (for 2 minutes. The homogenized material was then poured into 250 ml conical flask and placed into the shaker (for 12 hrs continuous shaking. After shaking, the slurry was filtered through a Buchner funnel with suction. The flask and filter cakes were rinsed with 25 ml of hexane each. The filtrate was then transferred into 250 ml round bottom flask and was dried to 5 ml by evaporation using a rotary vacuum evaporator. The concentrated filtrate was then transferred into 500 ml separatory funnel making 10 ml in volume. Around 20 ml methanol was added with 10 ml filtrate and shaked vigorously for 5 minutes. After shaking, the separatory funnel was set on stand and kept undisturbed for 5 minutes. Then the clear part of the solution from the bottom of the separatory funnel was collected in a vial which was then centrifuged and supernatant was collected for injection. The concentrated extracts were subjected to analysis for detection and quantification of pesticide residue in samples:

Source of Information: Annual Report 2012-2013, Entomology Division, BARI , Gazipur

Article Link (Online Journal):

Funding Source:

1.	Budget: 2,00000.00

Total Budget (Tk.) : 2,00000.00

Achievement/Findings:

The quantities were above MRL up to 8 DAS for fenitrothion in cauliflower; 7 DAS for fenitrothion in bean, brinjal, stem amaranth and red amaranth; 6 DAS for fenitrothion in cabbage and chilli; 5 DAS for fenitrothion in tomato; 4 DAS for cypermethrin in red amaranth and 3 DAS for cypermethrin in yard long bean, chilli and stem amarenth. At 10 DAS, no residue was detected from any of the tested samples except fenitrothion in cauliflower and cypermethrin in yard long bean. The determined PHI were 9 DAS for fenitrothion in cauliflower; 8 DAS for fenitrothion in bean, brinjal, stem amaranth and red amaranth; 7 DAS for fenitrothion in cabbage and chilli; 6 DAS for fenitrothion in tomato; 5 DAS for cypermethrin in red amaranth and 4 DAS for cypermethrin in yard long bean, chilli and stem amaranth.

Knowledge Management: Report/Proceedings