Dr. Mahbuba Begum
Senior Scientific Officer
TCRC, Joydebpur, Gazipur
M. Hossain, N. Nahar, K.A. Kader and M. Iqbal Hossain
Scientists
BARI
An experiment was conducted to characterize common scab causing pathogen of potato. Isolation was done from three varieties i. e., Asterix, cardinal and Diamant. A total of 10 (Ten) isolates of Streptomyces spp. were isolated from three varieties. Three isolates (A1, A2 and A3) from Asterix, three isolates (C1, C2 and C3) from cardinal and four isolates (D1, D2, D3 and D4) from Diamant were isolated as Streptomyces spp.
Characterization, Common Scab, Pathogen, Potato
Pest Management
To identify and characterize the common scab causing pathogen for taking proper control measure.
Plant material
Three potato varieties such as Diamant, Cardinal and Asterix were selected for the isolation of common scab pathogen. Tubers were collected from these varieties with typical symptoms of common scab. The tubers were stored in paper bags at 4°C during several weeks until the start of the experiment.
Isolation of Streptomyces spp.
Dilution method was used for isolating Streptomyces spp. from potato tubers. Tubers were washed under running tap water and than surface sterilized with 10% chlorox solution for 3 min and rinsed thrice with sterile water. The corky layer of each lesion from typical common scab symptom was lifted aseptically and about 5 mm3 of straw colored tissue directly underneath the layer was removed by a sterile scalpel and homogenized in sterile water (1.0 g/10 ml) in a mortar with pestle. One milliliter of the homogenate was mixed with 9 ml of phenol-water suspension (1:140). Three times diluted homogenate were plated on Yeast malt extract (YME-ISP medium 2) agar medium (pH 7.0). Plates were incubated at room temperature for 4-14 days under natural light. Bacterial colonies developing from the plant material were identified by microscopic observations and purified at least twice by serial transfers on YME. All the isolates were tested according to the International Streptomyces project (Shirling and Gottlieb, 1966)
Annual Report-2010-11, TCRC
40,000/-
Common scab pathogen isolated was Streptomyces spp. from three varieties. Three isolates (A1, A2 and A3) from Asterix, three isolates (C1, C2 and C3) from cardinal and four isolates (D1, D2, D3 and D4) from Diamant were isolated.
Report/Proceedings