Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Effect of Growth Regulators on Callus Formation and Plantlet Regeneration of Papaya

M. Mahmuda Khatun
Senior Scientific Officer
Biotechnology Division,BARI,Gazipur-1701

D. Khanam
Senior Scientific Officer
Biotechnology Division,BARI,Gazipur-1701

ASMMR Khan
Principal Scientific Officer
Biotechnology Division,BARI,Gazipur-1701

M. Al-Amin
Principal Scientific Officer and Head
Biotechnology Division,BARI,Gazipur-1701

Abstract/ Executive Summary:

Immature seeds and in vitro grown leaf, hypocotyl,root and leaf petiole segments were cultured in medium with different concentrations and combinations of growth regulators to establish a suitable regeneration protocol. Maximum callus formation was observed in 10 mg/l 2,4-D using immature seeds while hypocotyl segments produced maximum callus formation (81%) in the medium with combination of BAP, NAA and GA3. For shoot formation, 0.08 mg/l each of BAP + NAA and 3.7mg/l GA₃ performed better than other treatments. In different concentrations of IAA, only 10-30% shoots produced root. The maximum numbers of roots were recorded from 1.5 mg/l IAA concentration.

Keywords: Papaya, growth regulators, callus, regeneration

Location: Laboratory of Biotechnology Division, BARI, Gazipur-1701

Start Date: 25.01.2009

End Date: 12.07.2010

Program Area: Crop-Soil-Water Management

Commodity: Papaya

Objectives:

To find out the response of different growth regulators and determines their response to embryogenic callus formation and plantlet regeneration

Methodology:

Immature seeds were used for callus formation in two different basal media namely MS and N⁶. The seeds were washed with running tap water with jet trix for 30 min. After that the seeds were sterilized by Mercuric Chloride with two drops of Twin 20 and washed 3-4 times with sterilized distilled water.For callus formation, different concentrations of 2, 4-D were used in the medium. The in vitro grown explants were cultured in medium supplemented with combinations of BAP, NAA and GA3. Cultures were incubated at 25 ± 1⁰C with complete darkness in case of immature seeds. After callus formation, they were transferred to regeneration medium and kept in 16 hours photoperiod with a light intensity of 2000 lux under cool white fluorescent tubes.In other explants, they were incubated at light condition.The p^H of all media was adjusted to 5.8 before autoclaving. The media were autoclaved at 121⁰C and 1.06 kgcm^-2 pressure for 20 minutes.

Source of Information: Research Report (2009-2010)of Biotechnology Division, BARI,31 August, 2010

Article Link (Online Journal):

Funding Source:

1.	Budget: 30,000/-(Thirty thousand)

Total Budget (Tk.) : 30,000/-(Thirty thousand)

Achievement/Findings:

Result:

Callus formation and shoot development: The highest callus formation was noticed in 10 mg/l 2,4-D using immature seeds. On the other hand, hypocotyl segments produced maximum callus formation (81%) in the medium with combination of BAP, NAA &GA₃. When calli were transferred to regeneration medium, only embryogenic calli turned to greenish and produced mass of embryogenic calli. The calli were then divided into small pieces and placed in different concentrations and combinations of auxin and cytokinin. Result revealed that 0.08 mg/l each of BAP + NAA and GA₃performed better for shoot formation and development.

Root formation: For root initiation, in vitro grown shoots were transferred to root induction media.Different concentrations of IAA were used for rooting.Results showed that only 10 - 30 % shoots produced root in different concentrations of IAA. The highest percentage(30%) of roots was recorded in 1.5 mg/l IAA. On the other hand, control treatment did not produced any root. Root initiation ranged from 11 -30 days where early root formation (11 days) was observed from1.5 mg/l IAA and the maximum number of roots (5.3/plantlet) was also observed from the same concentration.

Knowledge Management: Report/Proceedings