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In Vitro Cultur of Orchid

M. Mahmuda Khatun
Senior Scientific Officer
Biotechnology Division, BARI, Gazipur-1701

H. Khatun
Research fellow (MS student of Biotechnology)
BAU, Mymensingh

D.Khanam
Senior Scientific Officer
Biotechnology Division,BARI, Gazipur-1701

M.A.Malek
Senior Scientific Officer
Plant Genetic Resources Centre,BARI, Gazipur-1701

M.Al-Amin
Principal Scientific Officer
Biotechnology Division,BARI, Gazipur-1701

Abstract/ Executive Summary:

The experiment was conducted to investigate the effect of different plant growth regulators on growth and development of plantlets from PLBs of orchid. Among the three growth regulators, 1.0 mg/l IAA showed the maximum weight of PLBs (12.150g),maximum width of leaf(0.733 cm), and maximum number of rooted plantlets (9,330). However, for root number, 2.0 mg/l IAA was found for better root formation (8.417)Whereas,highest root length (2.500 cm), highest fresh weight of single shoot (0.253 g) and maximum number of regenerated plantlets were obtained from 0.5 mg/l IAA.

Keywords: Orchid, Dendrobium, Hybrid, In vitro culture

Location: Laboratory of Biotechnology Division,BARI, Gazipur-1701

Start Date: 18.02.2006

End Date: 15.03.2007

Program Area: Crop-Soil-Water Management

Commodity:

Objectives:

To observe the effect of BAP, NAA and IAA on plantlet regeneration and the subsequent growth and development of hybrid Dendrobium

Methodology:

The PLBs were used as planting materials. MS medium was used with various concentrations of BAP, NAA and IAA. Experiments were carried out in a completely randomized Design with three replication. Data were collected for weight of PLBs, height of shoot, number of leaves per plantlet, length of leaves, width of leaves, fresh weight of single shoot, number of rooted plantlets per sample, number of roots per plantlet, number of regenerated plantlets. The colllected data were subjected to analysis of variance and means were separated by DMRT.

Source of Information: Journal: Bangladesh J. Agril.Res.33(2):319-325,2008

Article Link (Online Journal):

Funding Source:

1.	Budget: 30,000/-(Thirty thousand)

Total Budget (Tk.) : 30,000/-(Thirty thousand)

Achievement/Findings:

Result:

In vitro PLBs were cultured in MS medium supplemented with different concentrations of BAP, NAA and IAA. Among the three growth regulators, 1.0 mg/l IAA showed the maximum weight of PLB, maximum width of leaf and maximum number of rooted plantlets. Maximum height of shoot was obtained from 0.5 mg/l BAP. On the other hand, 2.0 mg/l IAA was found for better root formation whereas, highest root length, highest fresh weight of single shoot and maximum number of regenerated plantlets were obtained from 0.5 mg/l IAA.

Knowledge Management: Journal