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Screening for Cold Tolerance at Seedling Stage.

Dr. Serajul Islam
CSO and Head
Plant Physiology Division, BRRI, Gazipur

Md. Sazzadur Rahman
Scientific officer
Plant Physiology Division, BRRI, Gazipur

Abstract/ Executive Summary:

The study was conducted at BRRI, Gazipur. Objective of the experiment was to identify advance-breeding material which can tolerance low temperature at seedling stage. Eighteen advanced breeding lines (H3798, BR4726-2B-6-1-J₄ ,BR6734-11-1-2, BR6840-46-1-2, BR6618-1-2-1, BR6491-6-B-10, WAITA-4, BR6470--3-2-5, MT 6, BR6460-247-1-1-2, BR6342-69-5-3, IR61355-3B-11-2-2, PR26768-PJ(T)4C-18-8-2-1, VND 95-20, BR6484-21-7-3, BR6481-8-6-2, BR6619-19-12-3, BR6475-16-B-2) were selected as cold tolerant at seedling stage.

Keywords: Screening, Cold tolerance, Seedling stage, BRRI dhan36

Location: Plant Physiology Division, BRRI, Gazipur

Start Date: 00-07-2004

End Date: 00-06-2005

Program Area: Variety and Species

Commodity: Rice

Objectives:

To identify advance-breeding material which can tolerance low temperature at seedling stage.

Methodology:

The study was conducted at BRRI, Gazipur. Sprouted seeds of 36 advance breeding lines and 2 check varieties namely HbjBIV and BRRI dhan36 were sown on January 12, 2005 in netted bed attached with styro-foam sheet floating in a tray containing full strength cultural solution. The pH level of cultural solution was maintained at 5 thought out the experiment period. Each entry was sown in a line and 20 seedlings in each line were maintained. On February 13, 2005 seedling height, root length, root number, leaf number (stage), shoot dry weight and percent mortality were recorded. Seedling strength (mg/cm) were calculated using following formula: Strength (mg/cm) = Shoot wt (mg)/shoot height (cm).

Source of Information: Proceedings of the BRRI Annual Internal Review for 2004-2005 (Held on 18-22 December 2005). Bangladesh Rice Research Institute (BRRI), Gazipur -1701.

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Seedling strength: Most of the advanced breeding lines attained seedling strength of more than 3.00 mg/cm. visually stronger the seedling better was the survival at low temperature. HbjBIV had the seedling strength 3.39 mg/cm while BRRI dhan36 had the seedling strength 2.51 mg/cm. Screening for cold tolerance: Seedlings were screened following the scoring system mentioned above. The mean score was considered for the selected of a particular advance breeding line. Advance breeding line having a mean score of <5.67 were considered as tolerant and better BRRI dhan36.

Knowledge Management: Report/Proceedings