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Identification and Utilization of Qtls from Rice Wild Relatives for High Yield Through Use of Microsatellite Markers

Dr. Md Enamul Hoque
PSO and Head
Biotechnology Division, BRRI

Dr. Jannatul Ferdous
SSO
Biotechnology Division, BRRI

Abstract/ Executive Summary:

The popular varieties BRRI dhan28 and BRRI dhan29 were used as recipient parents and Oryza rufipogon Griff. (accession number 103404 and 105890) were used as donors. A total of 430 simple sequence repeat (SSR) markers were used to identify polymorphism between the parents. Among them 102, 108, and 89 primers were identified to use for genotyping of BRRI dhan28/Oryza rufipogon (Ac.no.105890), BRRI dhan28/Oryza rufipogon (Ac.no.103404), and BRRI dhan29/Oryza rufipogon (Ac.no.103404) crosses, respectively. Three BC2F2 populations were developed through backcross breeding method. A total of 657 individuals of three BC2F2 generations (238, 210 and 208 progenies of BRRI dhan28/Oryza rufipogon (Ac.no.105890), BRRI dhan28/Oryza rufipogon (Ac.no.103404), and BRRI dhan29/Oryza rufipogon (Ac.no.103404), respectively) were grown in the field. Phenotypic data on yield and yield component of all populations was recorded. Leaves from each plant were collected for DNA isolation as well as genotyping. For QTL analysis, 51, 6 and 12 polymorphic SSR markers were amplified for genotyping of 238 individuals of BRRI dhan28*3/O. rufipogon (Ac. No. 105890), 210 individuals of BRRI dhan28*3/Oryza rufipogon (Ac.no.103404) and 208 individuals of BRRI dhan29*3/Oryza rufipogon (Ac.no.103404) populations, respectively). QTL analysis will be performed by using QGene version4.3 software.

Keywords: QTLs Identification, Wild rice, Backcross, Population, SSR markers

Location: BRRI, Gazipur-1701

Start Date: 00-04-2010

End Date: 00-12-2013

Program Area: Variety and Species

Commodity: Rice

Objectives:

i. Identify and Introgress high yield QTLs to enhance yield

ii. Broaden the genetic base of modern rice varieties

iii. Strengthen the capacity of biotechnology laboratory of BRRI

Methodology:

Backcross: Three advanced backcross populations BC2F2 were developed through backcross breeding method. BRRI dhan28 and BRRI dhan29 were used as recurrent parent. Oryza rufipogon (Ac.no.103404) and Oryza rufipogon (Ac.no.105890) were used as donor parents. Two accessions of O. rufipogon served as a male parent for developing F₁s from the cross between BRRI dhan28/Oryza rufipogon (Ac.no.103404), BRRI dhan29/Oryza rufipogon (Ac.no.103404) and BRRI dhan28/Oryza rufipogon (Ac.no.105890). F₁ plants were grown in net house and three most vigorous F₁ plants were backcrossed to BRRI dhan28 and BRRI dhan29 (as male) to generate BC1F1 seeds. Second round of backcrossing was carried out with BRRI dhan28 and BRRI dhan29 to produce BC2F1 seeds. BC2F1 seeds were grown in the fields and best plants were selected based on phenotype and BC2F2 seeds were harvested individually. *Parental polymorphism and QTL analysis: *Phenotyping: *Genotyping:

Source of Information: Proceedings of the BRRI Internal Review Report, 2011-12

Article Link (Online Journal):

Funding Source:

1. World Bank	Budget: 68, 68,120.00
2. Bangladesh Agricultural Research Council	Budget: 68, 68,120.00

Total Budget (Tk.) : 68, 68,120.00

Achievement/Findings:

*Development high yield potentials of advance lines having yield enhancing QTLs for over existing varieties. *If the target high yield is achieved a tremendous socioeconomic impact is expected through increased food production and net high return. The country will be self reliant in food and potential farmers will sale their extra product followed by an elevated income and poverty reduction.

Knowledge Management: Report/Proceedings