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Effects of L-lysine on Growth and Histological Changes in Liver and Intestine of Gift Strain Oreochromis Niloticus

JINAT MAHBUB
Department of Fish Biology & Genetics, Sylhet Agricultural University, Sylhet, Bangladesh

MOHAMMED MAHBUB IQBAL
Department of Fish Biology & Genetics, Sylhet Agricultural University, Sylhet, Bangladesh

MD. SAKHAWAT HOSSAIN
Department of Aquaculture, Sylhet Agricultural University, Sylhet, Bangladesh

SOHEL MIAN
Department of Fish Biology & Genetics, Sylhet Agricultural University, Sylhet, Bangladesh

MD. NAIMUDDIN JABED
Department of Fish Biology & Genetics, Sylhet Agricultural University, Sylhet, Bangladesh

A.K.M. MUNZURUL HASAN
Department of Fish Biology & Genetics, Sylhet Agricultural University, Sylhet, Bangladesh

Abstract/ Executive Summary:

Four experimental diets were used for four treatments viz.T1 (SD+1.95% lysine of diet), T2 (SD+2.05% lysine of diet), T3 (SD+2.15% lysine of diet) and T4 (Control, SD+0.00% lysine of diet) with each having three replications to understand the effects of lysine on growth, histological changes in liver and intestine of GIFT strain of tilapia (Oreochromis niloticus). Forty-eight days old tilapia fry were stocked in experimental cemented tanks and fed twice a day with experimental diets containing different doses of L-lysine for 40 days from May to June 2016 and then the growth and health status of experimental fish were checked at every 10 days interval. The mean body weight treatment T2fish (13.04±0.3 g) was significantly higher (p<0.0001) followed by T3(10.58±0.14g), T1(9.86±0.07 g) and T4, (7.79±0.05 g), respectively. In addition, the body length was also increased 8.63±0.81 cm with T2 treatment followed by 8.59±0.08 cm, 8.42±0.12 cm and 8.1±0.06 cm with T3, T1and T4, respectively. SGR of fish were 2.41±0.004, 2.52±0.04, 245±0.07 and 2.07±0.04% in T1, T2, T3 and T4, respectively. Histology of liver and intestine was carried out with T1, T2, T3 and T4 treatment where the microvillus fold and height in the intestine were comparatively higher in T2, but there were no vacuoles and infiltrations in T2 compared to T1, T3 and T4. The present findings revealed that 2.05% supplementary lysine for tilapia might be a good promoter for their growth and culture.

Keywords: Lysine, GIFT, Histological changes

Location: Fish Biology and Genetics Department, Sylhet Agricultural University, Bangladesh

Start Date: 00-05-2016

End Date: 00-06-2016

Program Area: Variety and Species

Commodity: Tilapia

Objectives:

To investigate the effect of lysine on the growth performance and observe histological changes in the liver and intestine of tilapia.

Methodology:

Study area: Rectangular-shaped twelve cemented tanks facilitated to supply water from motor were used for the study in the laboratory of Fish Biology and Genetics Department, Sylhet Agricultural University for 40 days from 12th May to 22nd June 2016. Sample collection: Forty-eight days old fries of tilapia were collected from Pirojpur Fish Nursery in Sylhet district and acclimated to the laboratory conditions for 2 days prior to feeding trial for their adaptation to experimental diet. Experimental design: The experiment was conducted in four treatments each with three replications having almost the same sized 50 experimental fish. The treatments have been designated as T1(1.95% lysine), T2(2.05% lysine), T3(2.15% lysine)and T4 (0.00 % lysine), respectively. Diet preparation and feeding schedule: Supplemental diet was prepared according to Hasan (2015) with minor modifications. The feed was applied carefully twice a day during the 40 days experimental period. Half of the ration was supplied at 9.00 am and the remaining half was supplied at about 5.00 pm. Fish were fed at a rate of 20% of their body weight. Then the rate was gradually reduced to 12%. Physico-chemical parameter: Water quality parameters such as water temperature, pH, dissolved oxygen (DO), alkalinity, the concentration of ammonia, nitrite, nitrate and water transparency were measured at every 10 days interval. Temperature and DO were measured by a digital DO meter (YSI model 58) and pH with a pH electrode (Jenway, model 3020). Before taking a measurement, pH meter was properly adjusted with buffer solution (pH-7). Water quality parameters (temperature 25-29°C, dissolved oxygen≥ 6 ppm, and pH7- 7.5) were recorded between 08.00-09.00 am. Other parameters were monitored by HACH kit. Histological observation of liver and intestine: Histological study of the liver and intestine of tilapia was conducted according to the standard protocol. Briefly, the samples were kept in Bouin’s fixatives for 12-24 hours. Then the fixed specimens were processed to wax embedded blocks using a vacuum infiltration processor and standard protocols (Jabed 2013). Sections were cut at 3-5 μm on a motorized rotary microtome, mounted on glass slides, dried and stained with hematoxylin and eosin (H&E). Stained sections were observed under a compound light microscope and photographed by using an attached digital camera (Olympus Xcam- Alpha, Germany). Statistical analysis: Finally, the collected data were analyzed statistically by using IBM SPSS statistics, version 19.

Source of Information: Bangladesh J. Fish. (2018) 30(1) : 27-35

Article Link (Online Journal):

Funding Source:

1.

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The results from the present study confirm the effects of lysine on the performances of GIFT strain O. niloticus. Supplementation of lysine also enhanced the feed efficiency of tilapias as well as promote the development of the intestine and liver. Lysine plays a significant role in increasing body length and weight. This also helped in liver and intestine weight and the development of the experimental fish. In respect of body length and weight, liver weight and intestine weight, SGR, HIS, and VSI T2(2.05% lysine of diet) showed the best performance whileT3(2.15% lysine) showed intermediate performance (less than T2 but higher than T1 and T4). This might be due to the overdose of lysine. Thus, from the above findings, it can be concluded that incorporation of 2.05%lysine in tilapia feed may improve the growth, liver and intestinal health status of GIFT strain of tilapia which will ultimately help to increase the tilapia (GIFT strain) production.

Knowledge Management: Journal