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Identification of Certain Bioactive Compounds with Anthelmintic Properties in Azadirachta Indica and Clerodendrum Viscosum

Md. Nuruzzaman Munsi
Goat and Sheep Production Research Division, BLRI, Savar, Dhaka, Bangladesh

Md Ershaduzzaman
Farming System Research Division, BLRI, Savar, Dhaka, Bangladesh

Sonia Akther
Corresponding author:
Goat and Sheep Production Research Division, BLRI, Savar, Dhaka, Bangladesh

Md. Zakir Hassan
Animal Health Research Division, BLRI, Savar, Dhaka, Bangladesh

Md. Mamunur Rahman
Sheep Development Project, BLRI, Savar, Dhaka, Bangladesh

Milon Mondal
Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh

Abstract/ Executive Summary:

This study was undertaken to detect certain bioactive compounds with anthelmintic properties in Azadirachta indica and Clerodendrum viscosum by using high-performance liquid chromatography (HPLC). From the HPLC analysis, A. indica showed peak retention time which was similar to standard phenolic compounds including tannic acid (A. indica retention time 3.270 min, STD retention time 3.271 min) and pyrogallol (A. indica retention time 3.948 min, STD retention time 3.795 min). Benzoic acid (C. Viscosum retention time 6.092 min, STD retention time 6.067 min), tannic acid (C. Viscosum retention time 3.322 min, STD retention time 3.271 min) and quercetin (C. Viscosum retention time 4.967 min, STD retention time 4.222 min) was detected in leaf part of C. viscosum. Most of these ingredients have well-known anthelmintic roles. Thus, it can be concluded that Azadirachta indica and Clerodendrum viscosum leaves contain bioactive compounds with anthelmintic properties.

Keywords: Bioactive compounds, Azadirachta indica, Clerodendrum viscosum, HPLC analysis

Location: Savar upzilla, Bangladesh

Start Date: 00-11-2016

End Date: 00-12-2016

Program Area: Development of Host and Medicinal Plants

Commodity: Neem, Biological

Objectives:

To isolate and characterize the bioactive phytochemical compounds from methanol extract of the plant with the help of the High-Performance Liquid Chromatography (HPLC) technique.

Methodology:

Collection of plants: The plant of A. indica and Clerodendrum viscosum were collected from different areas of Savar Upazila, Bangladesh during the month of November-December 2016. Extraction of the plant: The collected plant leaves were sun-dried. Then they were heated through Oven to be fully dried at below 40ºC for two days. The fully dried leaves were then ground to make them powder by the help of a suitable grinder. The whole powders were extracted by cold extraction with solvent methanol and kept for a period of three (03) days accompanying occasional shaking and stirring. The whole mixture then underwent a coarse filtration by a piece of clean, white cotton material. Then these were filtered through Whatman filter paper. The filtrate (methanol extract) obtained was evaporated by Rotary evaporator (Bibby RE-200, Sterilin Ltd., UK) at 5 to 6 rpm and at 68ºC temperature. It rendered a gummy concentrate of greenish-black color. The gummy concentrate was designated as crude extract. Then the crude extract was dried by freeze drier and preserved at 4ºC (Shobana et al., 2009). Phytochemical analysis: All the extracts were subjected to qualitative phytochemical screening to identify the presence of alkaloids, flavonoids, carbohydrates, gum, reducing sugars, saponins, steroids, tannins and terpenoids using the established methods of HPLC. Plants powder (5 g) was dissolved in 50 mL acidified deionized water (pH 2, achieved by the addition of 0.2 M HCl). The solution was passed through preconditioned C18 cartridges (3 mL 3 500 mg) purchased from Agilent Technologies. The cartridges were preconditioned by sequentially passing through 3 mL each of methanol and acidified water (pH 2) at a drop-wise flow rate. The aqueous extract solution (10 mL) was then applied to the preconditioned cartridges at a drop-wise flow rate to ensure the efficient adsorption of phenolic compounds. The adsorbed phenolics were then eluted from the cartridges with 1.5 mL of 90% v/v methanol/ water solution at a drop-wise flow rate. The entire extraction procedure was repeated three times. The eluent was collected and stored at 220C before HPLC analysis using an HPLC system

Source of Information: Asian Australas. J. Biosci. Biotechnol. 2018, 3 (2), 170-173; ISSN 2414-1283 (Print) 2414-6293 (Online)

Article Link (Online Journal): www.ebupress.com/journal/aajbb

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It can be concluded that Azadirachta indica and Clerodendrum viscosum leaves possess bioactive compounds with anthelmintic properties which have been detected accurately by HPLC.

Knowledge Management: Journal