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Study on Identification of Ploidy Level of Regenerated Plants Obtained from Cultured Wheat Anthers Using Length of Guard Cells

Md. Monirul Islam*
Discipline of Agrotechnology, Khulna University, Khulna 9208, Bangladesh

Mahtalat Ahmed
Discipline of Agrotechnology, Khulna University, Khulna 9208, Bangladesh

G.I. Ivanov
Department of Biotechnology, Kuban State Agrarian University, Krasnodar, Russia

P. Gain
Discipline of Agrotechnology, Khulna University, Khulna 9208, Bangladesh

Abstract/ Executive Summary:

A fast and reliable method for ploidy determination of regenerated Triticum aestivum from anther culture is described in the present work. The anthers of 47 hybrid combinations of winter wheat were cultured in vitro and the length of the guard cells was measured in regenerated plants. Thus, plants with a guard cell length of 32-46 mm were haploid, whilst plants with a guard cell length of 54-74 mm were diploid. Plants with a guard cell of 46-54 mm required further chromosome investigation for the exact determination of ploidy level.

Keywords: Guard cell; Ploidy level; Diploid; Haploid; Chromosome number

Location: At the laboratory of Cell Engineering and Biotechnology of Lukyanenko Agricultural Research Institute, Krasnodar, Russia.

Start Date: 00-00-1994

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Wheat, Plant

Objectives:

The objective of the present work was to develop an express-method for easy identification of ploidy level of regenerated plants without counting the chromosome number.

Methodology:

The present experiment was conducted in 1994 at the laboratory of Cell Engineering and Biotechnology of Lukyanenko Agricultural Research Institute, Krasnodar, Russia. Eight hundred twenty regenerated green plants obtained from the wheat anthers of 47 hybrid combinations were used as experimental materials. For the identification of haploid and diploid (produced by spontaneous doubling of chromosomes) plants, length of the guard cells of green regenerated plants was measured. For this purpose a small piece of leaf was dissected out from the middle portion of the second leaf and observed under microscope. From each plant, length of 25 guard cells was measured by the scale of microscope and at the same time the chromosome number of the cells of root tips were counted from the same plants. Potato-II (Chuang et.al., 1978) liquid medium and MS medium (Murashige and Skoog, 1962), both supplemented with 2, 4 D- 0.75 mgl-1 ; µ NAA- 0.1 mgl-1 ; kinetin- 0.75mgl-1 were used for callus induction and plant regeneration subsequently.

Source of Information: Khulna University Studies 2(1): 119-122

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The present research work has shown that regenerated wheat plant through in vitro anther culture with the guard cells length of 32-46 mm are haploid and 54-74 mm are diploid. Plantlets with guard cells of 46-54 mm required further chromosome investigation for exact determination of ploidy level, because regenerated plants belonging to this class were identified both as haploid and diploid.

Knowledge Management: Journal