Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Regeneration of Grass Pea (lathyrus Sativus L.) (2016-2017)

S. Ahmed
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. A. Y. Akhond
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. R. Kabir
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

S.Yesmin
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

Abstract/ Executive Summary:

Seedling-derived cotyledonary nods of BARI Khesari-3 were cultured in vitro on MS medium supplemented with varying concentrations of 6-benzyl amino purine (BAP). Direct shoot regeneration was observed from explants. The highest percentage of response (58.33%) was observed in T3 and the highest shoots/explant (13) was found in T4. The experiment will be continued.

Keywords: Grass pea, Pulses, BARI Khesari-3, Clean seeds, Healthy seeds

Location: Pulse Research Centre, Bangladesh Agricultural Research Institute, Bangladesh

Start Date: 00-00-2016

End Date: 00-00-2017

Program Area: Crop-Soil-Water Management

Commodity: Grasspea

Objectives:

To develop a standard and efficient regeneration protocol of some BARI released varieties of grass pea.

Methodology:

BARI Khesari-3 seeds were collected from Pulse Research Centre, Bangladesh Agricultural Research Institute. Clean and healthy seeds were washed with 70% ethanol and sterilized for 25 minutes in 60% (v/v) sodium hypochlorite containing 3-4 drops Tween 20 in a laminar airflow cabinet. Afterward, they were rinsed 4 times with sterile distilled water and transferred to ½ strength MS medium (Murashige and Skoog, 1962) for germination. Cotyledonary nodes were cultured on MS medium supplemented with varying concentrations of 6-benzyl amino purine (BAP). Explants were subcultured every 14 days on the same media and kept under same conditions. The experiment was set up in a completely randomized design (CRD) with four independent replicates.

Source of Information: Annual Research Report 2016-2017, Biotechnology Division, bARI Joydebpur, Gazipur, Bangladesh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The cotyledonary node explants showed direct shooting response for all treatments. The highest percentage of shooting (58.33%) was observed in T3 and the lowest percentage (44.44) was found in T2. T4 produced the highest no. of shoots/explant (13) where T1 produced the lowest shoot/explants (5). Saha et al. (2015) also found direct shoot regeneration from different explants of grass pea. Further study is needed using different explants and growth regulators.

Knowledge Management: Report/Proceedings