Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Bulblet Production of Hippeastrum (hippeastrum Hybridum) (2016-2017)

M. K. Jamil
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. M. Khatun
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. K. Hasan
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

S. C. Halder
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

D. Khanam
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

Abstract/ Executive Summary:

The study was undertaken with a view to develop a suitable and reproducible protocol for in-vitro propagation of Hippeastrum (Hippeastrum hybridum). Twin scales from healthy and disease free bulb were used as explant and cultured on MS media supplemented with different concentration of BAP and NAA along with a control. The response of the explant varies with different concentration of plant growth regulators. MS media supplemented with BAP performed better than NAA in all other parameters. However, the highest percentage (98.33%) of shoot formation, number of shoots (2.24), shoot height (8.73 cm), fresh weight of shoots (544.7 mg) and earliest shoot proliferation (47.33 days) per explant were found in T₂ treatment i.e. MS media supplemented with 0.5 mgL^-1 BAP.

Keywords: Flower, Hippeastrum, Hippeastrum hybridum, Medicinal plants

Location: Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur, Bangladesh

Start Date: 00-00-2016

End Date: 00-00-2017

Program Area: Crop-Soil-Water Management

Commodity: Medicinal Plants, Flower

Objectives:

To develop a suitable protocol for in vitro propagation of Hippeastrum (Hippeastrum hybridum) using different concentrations of growth regulators and make the plant materials available to the farmers for commercial cultivation.

Methodology:

Healthy and disease free bulbs of Hippeastrum were collected from Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Salna, Gazipur. The bulbs were surface sterilized with detergent for 20 minutes and washed with running tap water for 15 minutes. Then the bulbs were cleaned with sharp knife by cutting its basal roots and outer scales. After that the bulbs were cut into four pieces and kept for 30 minutes with Ridomil gold MZ 68 WG to remove the fungal contamination. Then the cut pieces of bulbs were sterilized with Jet and Trix and wash with running tap water for 5-6 minutes followed by 3-5 times washing with distilled water. Then the cut pieces of bulbs were sterilized with Clorox solution with 2 drops of Tween -20 for 3 minutes followed by 3-5 times washing with sterilized distilled water inside the laminar air flow cabinet.The dead tissue and the remaining portion of roots were cut off and the explant were prepared with two scales joined at the basal plate. The size of the twin scale were 1 x 2.5cm.The prepared explant were placed in MS media supplemented with different concentrations of BAP and NAA. All cultures were incubated at 25±1⁰C with a 16 h photoperiod (2000 lux) provided by cool white fluorescent tubes.

Source of Information: Annual Research Report 2016-2017, Biotechnology Division, BARI, Joydebpur, Gazipur, Bangladesh

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From the experiment it can be concluded that NAA has less effect than BAP on MS media for invitro bulblet production of hippeastrum by twin scales. However, BAP on MS media at 0.5 mgL^-1 performed better than all other treatments in case of shoot proliferation, percentage of shoot formation, shoot number, shoot height and fresh weight of shoots.

Knowledge Management: Report/Proceedings