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Study of Comparative Regeneration Efficiency of Different Potato Varieties (2016-2017)

M. M. Khatun
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. R. Kabir
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

D. Khanam
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M. A. Y. Akhond
Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

Abstract/ Executive Summary:

Internode explants of three BARI released high yielding potato varieties (BARI Alu-40, BARI Alu-41, BARI Alu-25) and one exotic (Maris Piper) varieties were cultured to compare their regeneration efficiency. Five different concentrations (T₀, T₁, T₂, T₃, & T₄) of Zeatin Riboside in combination with GA₃and IAA were used for regeneration of explants. Among the four varieties, the maximum responding variety was BARI Alu-41 and the best treatment combination was T₂ regarding percent shoot response, shoot height and leaf number.

Keywords: Potato varieties, Potato, High yielding potato, Shoot response, Shoot height, Leaf number

Location: Biotechnology Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

Start Date: 00-00-2016

End Date: 00-00-2017

Program Area: Variety and Species

Commodity: Potato

Objectives:

To compare regeneration efficiency of potato varieties.

Methodology:

One exotic (Maris Piper) and three BARI released high yielding potato varieties viz. BARI Alu-40 (4.45 W), BARI Alu-41 (5.183) and BARI Alu-25 (Asterix) were used for this study. Tissue culture plantlets of those three varieties were collected from Tuber Crop Research Centre (TCRC) of BARI. Five plantlets of each variety were obtained. To increase the number of plantlets, those materials were further grown on modified Murashige and Skoog basal medium by node cutting. When sufficient explants were obtained then the experiment was set up. For regeneration, internode explants were cut into small pieces and placed in modified MS media containing different concentrations of Zeatin Riboside, GA₃ and IAA. The P^H of all media was adjusted to 5.8 before autoclaving. The media were autoclaved at 121⁰C for 20 minutes. All cultures were incubated at 25 ± 1⁰C and kept in 16/8 hours light/dark photoperiod with a light intensity of 2000 lux under cool white fluorescent tubes.

Source of Information: Annual Research Report 2016-2017, Biotechnology Division, BARI, Gazipur

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In the above experiment, result showed that the variety BARI Alu-41 performed better than other varieties. The T₂ treatment was better for shoot regeneration regarding percent shoot response, shoot height and leaf number compared to other treatments.

Knowledge Management: Report/Proceedings