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In- Vitro Propagation of Guava (psidium Guajava L.) (2018-2019)

M. K. Jamil
Pomology division of HRC, Gazipur.

Abstract/ Executive Summary:

The study was undertaken with a view to develop a suitable and reproducible protocol for in-vitro propagation of guava (Psidium guajava L.). Shoot tips from healthy and disease free plants of BARI peyara-2 were used as explant and cultured on MS media supplemented with different concentration of BAP and Kinetin along with a control. The response of the explant varies with different concentration of plant growth regulators. MS media supplemented with BAP performed better than Kinetin in all parameters. However, the highest survival percentage (100 %) of explant were found in T₂ treatment i.e. MS media supplemented with BAP at 0.5 mgL^-1 and the maximum number (2) of explant turns into greenish color in T₃, T₄ and T₅ treatments of BAP.

Keywords: Guava, Fruit, Healthy seedlings, Disease- free seedlings, BARI Peyara-2

Location: Pomology division of HRC, Gazipur, Bangladesh.

Start Date: 00-00-2018

End Date: 00-00-2019

Program Area: Crop-Soil-Water Management

Commodity: Guava

Objectives:

To develop an efficient in vitro propagation protocol for rapid and mass propagation of guava and make the plant materials available to the farmers for commercial cultivation.

Methodology:

Shoot tips from three months old healthy and disease- free seedlings of BARI Peyara-2 were collected from the nursery of pomology division of HRC, Gazipur. The shoot tips were surface sterilized with detergent for 20 minutes and washed with running tap water for 30 minutes. Then the shoots were defoliated with sharp knife by cutting its basal leaves and twigs. After that the shoot tips were cut into 3-4 cm in size and kept for 30 minutes with Ridomil gold MZ 68 WG and krosin to remove the fungal and bacterial contamination. Then the explants were dipped in Jet and Trix solution and washed with distilled water for 5-6 times. Then the shoot tips were sterilized with Clorox solution (60%) with 2 drops of Tween -20 for 10 minutes followed by 3-5 times washing with sterilized distilled water and again dip in 70% (v/v) ethanol for 2 minutes followed by 5-6 rinses in sterile distilled water inside the laminar air flow cabinet. After sterilization, the explants were prepared at 1.5-2.0 cm in size. The prepared explant were placed in MS media supplemented with different concentrations of BAP and Kinetin. All cultures were incubated at 25±1⁰C with a 16 h photoperiod (2000 lux) provided by cool white fluorescent tubes.

Source of Information: Annual Research Report 2018-2019, Fruit Research Farm of HRC, BARI, Gazipur

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From the experiment, it can be assumed that Kinetin has less effect than BAP on MS media for shoot proliferation of guava by shoot tip. However, BAP on MS media at 0.5 mgL^-1 performed better than all other treatments for preliminary responses of the explant. The experiment is now on going. So it needs to be continued for the next year to obtain the final result of the experiment.

Knowledge Management: Report/Proceedings