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Kanamycin Potential of Papaya Fruit Stem Exudate in E. Coli

Sharif M. Shaheen
Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh

Md. Kamrul Hasan
Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh

Abstract/ Executive Summary:

Fruit stem exudate of a Papaya was collected and antibacterial activity was evaluated against E. Coli colony culture. The activity was done by an agar well diffusion method. Two concentrations of crude papaya exudate of 300 and 500 µg/disc were adsorbed and allowed for diffusion. A kanamycin standard of the 30µg / disc was compared with the above two concentrations of papaya exudate and found nonsignificant difference between the zone of inhibitions. The results potentiated papaya fruit stem exudate to the kanamycin type of antibiotic, which might be a potential natural antidote and antibiotic for future decades of medicinal applications.

Keywords: Kanamycin, Papaya fruit stem exudate, Zone of inhibition,

Location: Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh

Start Date:

End Date:

Program Area: Resource Development and Management

Commodity: Medicinal Plants

Objectives:

Here, in order to explore the antibacterial activity we examined the fruit stem exudate of papaya on E. Coli as a preliminary proof of natural antibiotic potentiality with that of kanamycin, a naturally occurring antibiotic.

Methodology:

Materials: Agar, petri dish was purchased from the local market. Kanamycin was used as analytical grade. E. Coli DH α strain was cultured from glycerol stock in LB media, which was donated by the microbiology department of the Rajshahi University. Bactotryptone, Yeast extract, NaCl and Paper disc of Hi-media method was also purchased from the local market. Methods: Papaya fruit stems exudate collection: Papaya fruit stem is the cylindrical stem bark between the fruit and tree. The fruit stem bark was scratched well with sharp knife and allowed to exudate. The milky white exudate was collected carefully avoiding any kind of contamination. E. Coli culture, inoculation and antibacterial activity: LB media was prepared using bactotrypton, yeast extract, NaCl and E. Coli from 80% glycerol stock was moved to inoculate the LB. The inoculated LB was then incubated at RT for an hour. Then antibacterial activity was done using agar well diffusion method. For this 20ml of sterile Mueller-Hinton Agar (Himedia) was poured into a sterile autoclaved petri dish. After solidification, the sterile cotton swab was dipped into the bacterial culture. The entire agar surface of each plate was evenly inoculated by swabbing. Two concentrations of Papaya exudates 300 µg/disc and 500 µg/disc were used and allowed to diffuse for at least 45 minutes. The plates were then incubated at 37°C for 24 hrs. Triplicate plates were prepared for each treatment and the average zone of inhibition excluding well was recorded. The antibacterial potential of exudate was compared with that of standard Kanamycin (30μg/ disc) with paper disc (Hi-media) method.

Source of Information: Int J Pharm Sci & Scient Res.1:1, 17-20.

Article Link (Online Journal):

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Total Budget (Tk.) :

Achievement/Findings:

Here we have shown that papaya fruit stem exudate has got the same or more potential in respect of antibacterial activity of a naturally occurred antibiotic Kanamycin, where papaya doesn’t show any toxicity in biological system so far reported elsewhere. Our results suggest to explore the biological activity of papaya fruit stem exudate in the days ahead.

Knowledge Management: Journal