Three varieties of cotton seeds, namely HC-1, HC-2 and HC-3 were collected from Cotton Development Board (CDB), Khamarbari, Farmgate, Dhaka. The collected seed samples were kept in polythene bag with airtight container in two conditions, one in room temperature (25°C) and other in refrigerator at 4ºC temperature for subsequent use. Fungi associated with cotton seeds were isolated following ‘Blotter’ and ‘Tissue Planting’ methods (Shamsi et al. 2010). The experiment was conducted in the laboratory of Mycology and Plant Pathology, Department of Botany, University of Dhaka, Bangladesh. Identification of the isolated pathogenic fungi was determined following standard literatures (Thom and Rapper 1945, Benoit and Mathur 1970, Booth 1971, Barnett and Hunter 2000). Pathogenicity test of the isolated fungi were done following ‘seed inoculation technique’ (Chowdhury et al. 2015). Five fungicides with different active ingredients viz., Acrobat MZ (60% mancozeb and 9% dimethamorph), Autostin 50 WDG (50% carbendazim), Capvit 50 WP (copper oxychloride), Nativo 75 WP (tebuconazole and trifloxystrobin) and Thiovit 80 WG (sulphur) were collected from the Siddique Bazar, Gulistan, Dhaka. In vitro fungitoxicity of these fungicides at 100, 200, 300, 400 and 500 ppm concentrations was evaluated against Aspergillus flavus, Aspergillus niger (Type-I), Curvularia lunata, Fusarium moniliforme var. subglutinans, Fusarium sporotrichioides and Rhizoctonia solani. For each fungicide, a stock solution having the concentration of 10,000 ppm was prepared. The calculated amount of stock solution of a fungicide was supplemented with sterilized PDA medium to get the concentration of 100, 200, 300, 400 and 500 ppm, respectively. The concentrations of fungicides were expressed in terms of its active ingredients. In control set, required amount of sterilized water instead of fungicide solution was added to the PDA medium. Then 15 ml of medium was poured in each Petri plate and allowed them to solidify. Therefore, at the center of the plate 5 mm agar disk of test pathogen was inoculated. Three replications were maintained in each treatment. The plates were incubated at 25 ± 2°C in an incubator. The radial growth of control and treatment plates were measured at 5 days of incubation. Fresh leaves of five angiospermic plants, namely Adhatoda vasica Nees., Azadirachta indica A. Juss., Datura metel L., Aegle mermelos L. and Psidium gujava L. were selected for evaluating their efficacy on the radial growth of six pathogenic fungi isolated from hill cotton viz., Aspergillus flavus, Aspergillus niger (Type-I), Curvularia lunata, Fusarium moniliforme var. subglutinans, Fusarium sporotrichioides and Rhizoctonia solani. Leaves of the selected plants were collected from the Botanical Garden of Curzon Hall Campus and Kabi Sufia Kamal Hall, University of Dhaka. Leaves of each plant were thoroughly washed in tap water, air dried and then used for fresh extract preparation. Leaf extracts were prepared by crushing known weight of fresh leaves with distilled water in ratio of 1 : 1 (w/v). In this method, the requisite amount of the filtrate of each plant extract was mixed with PDA medium to get 5, 10, 15 and 20% concentrations. The medium thus prepared was poured into sterilized Petri plates and allowed to solidify. Each Petri plate was inoculated centrally with a 5 mm agar disc cut from the margin of actively growing culture of the test pathogens. In the control set, a Petri plate containing PDA medium with the requisite amount of distilled water instead of a plant extract was also inoculated with agar disc of the test pathogen in the same manner as described above. Three replications were maintained for both the experimental and control sets. The inoculated Petri plates were incubated at 25 ± 20C. The radial growth of the colonies of the test pathogen was measured after 5 days of incubation. The fungitoxicity of the fungicides and plant parts extracts in terms of percentage inhibition of mycelial growth were calculated by using the following formula: I =C T/C× 100; where, I = Per cent growth inhibition, C = Growth in control, T = Growth in treatment. The data were collected as inhibition percentage of the radial growth of the pathogen in mm in each replication and evaluated by analysis of variance (ANOVA) by using STAR statistical program and means were compared using DMRT.