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Evaluation of Insecticidal Activity of Ni (ii) Cystine Against the Red Flour Beetle (tribolium Casteneum)

Mst Jesmin Ara
Dept. of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.

J. A. Khanam
Dept. of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh.

M. Rakibuzzaman
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.

A. F. M. Jamal Uddin
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.

Abstract/ Executive Summary:

The study was accomplished at the Crop protection and Toxicology Laboratory, Department of Zoology, Rajshahi University to evaluate the insecticidal activity of Ni (II) Cystine against red flour beetle. The main aim of this research is to evaluate the insecticidal activity of Ni (ii) cystine against the red flour beetle. Residual film bioassay and fumigation bioassay test were done and four doses of stock solution viz. S₁: 2 ml, S₂: 1 ml, S₃: 0.5 ml and S₄: 0.25 ml used in this study. Among them Residual film bioassay with S1 showed strong activity against red flour beetle while fumigation test showed no activity on T. casteneum. Highest mortality (70%) found in film method and LD₅₀ found 0.62 mg cm^-2 in film bioassay test. Therefore, this study reveals that residual film method and Ni (II) cysteine has potential insecticidal activity against red flour beetle.

Keywords: Film bioassay, Fumigation, Red flour beetle, Mortality

Location: Crop protection and Toxicology Laboratory, Department of Zoology, Rajshahi University

Start Date:

End Date:

Program Area: Pest Management

Commodity: Insects, Insecticide

Objectives:

To evaluate the insecticidal activity of Ni (ii) cystine against the red flour beetle

Methodology:

Insecticidal activity of Ni (II) cystine has been evaluated on Tribolium castaneum by residual film bioassay and fumigation bioassay method. Probit mortality of Tribolium castaneum was found to be 5.43731, 4.85612, 4.27493 and 3.69375 after 24 hours of surface film treatment with Ni (II) cystine complex at dose 2ml, 1 ml, 0.5ml and 0.25ml respectively. From these observation, calculated LD₅₀ value have been found to be 0.6173 for Tribolium castaneum indicating significant insecticidal effect of Ni(II) cystine on these insects. Surface film method also used for determining the effect of Ni (II) cystine on Tribolium castaneum. Different strains of Tribolium castaneum (Herbst) cultures are maintained for about 15 years in the Crop protection and Toxicology Laboratory, Department of Zoology, Rajshahi University. From the cultures standard local strain of the beetles was collected for the present study. Red flour beetles were sorted out to start a fresh culture. Earthen pots, Beakers (500 ml), Petri dishes, Camel hairbrush, Filter paper were used for maintaining the culture. All the equipment were kept in an oven for sterilization about six hours at 60^°C. Mass cultures were maintained in earthen pot and subcultures in beakers with the food medium. The beakers were kept in an incubator at 30^°C ± 0.5^°C without light and humidity control. The cultures were checked in regular intervals and eggs and larvae were separated to increase properly. For continuously and huge supply of the beetle’s sub-cultures were maintained in the incubator. The wheat flour was used as the food medium for T. castaneum. For each strain about 500 beetles were placed in a 500-ml beaker containing food medium and kept in an incubator at 30^°C ± 0.5^°C. The eggs were then transferred to Petri dishes (90 mm) and incubated at the same temperature. After 3-5 days larvae hatched out in that conditions. Newly hatched larvae were then collected with a fine pointed camel hair- brush and then shifted to the fresh food medium for culture. Then larval instars determine and larval cultures were maintained in an incubator at 30°C ± 0.5°C without light and humidity control. Newly formed adults were collected in a small beaker with the help of a camel hair brash. With four replications, different doses of Ni-cyst were applied as 2, 1, 0.5 and 0.25 mg ml and the mortality was subjected to probit analysis. Probit Analysis is a specialized regression model of binomial response variables. The regression is a method of fitting a line to the data to compare the relationship of the response variable (Y) to the independent variable (X). The dead beetles in Petri dishes after 24 hours treatment. To carry this experiment by residual film method the adult red flour beetles were used. Ten beetles were used in each replication. The compound was weighed and diluted in alcohol to obtain doses in which mortality rate was between 10 to 90% for the 4 to 10 days old beetles. Four doses of stock solution viz. S₁:2 ml, S₂:1 ml, S₃:0.5 ml and S₄:0.25 ml used in this study. In each petridish 10 beetles were inoculated and S₅: control experiment was maintained with alcohol. The mortality of the beetles was recorded after 24 hours of treatment. The compound was tested for its fumigation activity to T. castaneum. For that two sets of experiments were done.

Source of Information: J. Biosci. Agric. Res. 21(01): 1762-1766, 2019.

Article Link (Online Journal): https://doi.org/10.18801/jbar.210119.215 1762

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From this experiment, we conclude that fumigation test compound Ni (II) cystine was not shown any activity to Tribolium castaneum while 2ml stock solution had strong activity against red flour beetle in bioassay test.

Knowledge Management: Journal