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Potato Germplasm Innovation Through Micro Tuberization Technique

M. Nasiruddin
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Maliha
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Humayra Armin
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

M. Rezaul Karim
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

M. Monzur Hossain
Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

In vitro micro tuber formation potentiality of potato was investigated to establish a rapid disease free seed production system as well as potato germplasm innovation. Micro tubers were induced to meristem derived in vitro grown shoots in culture bottles increasing the concentration of BA (6-Benzyl adenine), KIN (Kinetin) and sucrose in MS medium. KIN showed better performance than BA for micro tuber induction and among the three concentrations (6-10 mg/l) of KIN, 8 mg/l KIN was the most effective and more preferred concentration for micro tuber induction. It was observed that increase the level of KIN also increased the percentage of tuberization, number and weight of micro tuber/shoot. By increasing the concentration of sucrose increased the presentence (%) of in vitro tuberization. The media containing 60 g/l was found to be the best among three concentrations (40-70 g/l) of sucrose tested followed by 70 g/l sucrose for micro tuber induction for all four potato cultivars.

Keywords: Micro tuberization, BA (6-Benzyl adenine), Kinetin, Sucrose

Location: Potato germplasm bank of Plant Breeding and Gene Engineering Lab, Department of Botany, Rajshahi University

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Potato

Objectives:

1. To find out the best concentration for BA (6-Benzyl adenine), KIN (Kinetin) and sucrose for successful micro tuber production of potato.

2. To standardize the media for potato plant growth and micro tuber induction.

Methodology:

Disease free tissue cultured meristem derived plantlets of four potato (Solanum tuberosum) varieties-Lady rosety, Asterix, Shepody and Indurkani were used as primary sources of explant for in vitro tuberization which were obtained from the Potato germplasm bank of Plant Breeding and Gene Engineering Lab, Department of Botany, Rajshahi University.The plant growth regulators and additives were used for this experiment in different concentration was Benzyl adenine (BA), 6-Furfuryl amino purine or kinetin (KIN). - MS formulation was used in the present study. All chemical compounds including macro and micro nutrient, organic acid and inorganic acids, sugar, agar, KOH, 70% ethyl alcohol etc. The plant material and nutrient media were contained within some type of culture vessel; the whole constituting a culture system. The culture vessel such as bottle (12 x 5mm), conical flask (250ml, 1000ml), measuring cylinder glass rods, beaker, pipette pumps, rubber bands, filter paper, aluminum foils, marker pen, spirit lamp, forceps, needle, scalpels blade, firebox electronic balance, autoclave, pH meter, magnetic stirrer, Laminar airflow machine etc. were also used in the present experiment. MS medium was used for node culture and further tuberization. Sucrose was added at the rate of 40-70 g/l. Plant growth regulators were added separately to different media according to requirements. Agar was added at the rate of 6.5 g/l for the preparation of semi-solid MS medium. Stock solution of PGR (plant growth regulators), the nutrient media were used. To prepare the stock solution of any of these plant growth regulators, 10 mg of solid growth regulators were placed in to a clean test tube and then dissolved in specific solvent. The volume of the solution was then made up to 100 by adding distilled water. It was stored in a refrigerator in 4°C-6°C temperature for ready to use at any time. Data were collected as percentage of in vitro tuberization, Number of micro tubers/shoot, weight of micro tubers/shoot. All the counted data imputed in mean value with standard deviation (M±SD) and it was tested with DMRT technique.

Source of Information: International Journal of Biosciences | IJB |; ISSN: 2220-6655 (Print) 2222-5234 (Online); Vol. 3, No. 1, p. 135-141, 2013

Article Link (Online Journal): http://www.innspub.net

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The experimental results revealed that the acceleration of micro tuber formation varied by concentration, types of phyto hormone and genotypes. The results obtained in the present investigation showed that, there was no significant difference between 40, 60 and 70% sucrose but on average, 60% sucrose produced more micro tubers than 40, 70, and in control.

Knowledge Management: Journal