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Effect of Sources and Concentrations of Inoculum at Different Time on in Vitro Digestibility of Hay

S. Akhter
Department of Animal Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M.M. Hossain
Department of Animal Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

An investigation was made to study the effect of sources, concentrations of inoculum and different incubation period on in vitro organic matter digestibility (OMD) and dry matter digestibility (DMD) of hay. Faecal liquor (83 or 333 g/l saliva solution) and rumen liquor (50 or 200 mI/l saliva solution) were used as source of micro-organisms in in vitro fermentation. The dry matter digestibility (DMD) increases with increasing incubation time for both faceal (301, 590 and 638 g/kg for low and 359, 625 and 675 g/kg for high) and rumen liquor (274, 541, and 663 g/kg for low and 396, 604 and 734 g/kg for high). The Organic Matter Digestibility (OMD) increases with increasing incubation time for both faecal (259, 529 and 560 g/kg for low and 348, 579 and 670 g/kg for high) and rumen liquor (260, 506 and 619 g/kg for low. and 368, 583 and 717 g/kg for high). The interaction effect of sources, concentrations and time on OMD and DMD did not show any significant (P>0.05) effect. This experiment suggests further investigation with faecal liquor with extending incubation period to use as source of micro-organisms for in vitro digestibility assay.

Keywords: Digestibility, Hay, Dry matter, Organic matter, Concentration

Location: University of. Reading, U.K.

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Straw

Objectives:

To study the effect of sources concentration of inoculum and incubation time on in vitro digestibility of dry matter and organic matter of hay.

Methodology:

Layout of the experiment: The experiment was laid out in split design by placing inoculum source, concentration of faeces and rumen liquor allocated to the main plot, and time allocated to the split plot. Total number of observations (including blanks) were 128 for in vitro digestibility trial.Preparation of samples and chemical analysis: Ryegrass (Lolium perenne) hay was used in this experiment and collected from the Old House Farm (Animal Production Research Unit), University of. Reading, U.K. Hay sample was ground through a 1 mm dry mesh screen to perform in vitro studies as well as chemical analysis. The DM, Ash, N, ADF and NDF contents of the hay was determined following the procedure of AOAC (1984) and Goering and Van Soest (1970). Hay used had the following composition (g/kg DM): DM 870 g!/g; Ash 80; N 28; ADF 326; NDF 719.Diet of the animals used for faeces or rumen liquor collection: Three Jersey cows were used for faeces collection. Individual housing facilities were provided for all cows. Each animal was offered ad libitum ryegrass as their daily feed. The nitrogen, ADF and NDF contents of the hay were 25, 421 and 713 (g/kg DM) respectively. Ad libitum water and mineral block were also supplied to the cows. Three Suffolk cross fistulated wethers were used for rumen liquor collections. The sheep were naintained on ad libitum hay and concentrate (200 g/d). The nitrogen, ADF and NDF contents of the hay were 23, 414 and 715 (g/kg DM) respectively. Concentrate mixture had in vitro DMD of. 878 g!kg and N of 31.8 g/kg DM.

Faeces collection and liquor preparation: Faeces sample was collected from the rectum of cow at 0640 hrs and preserved in a prewarmed (39°C) vacuum flask until used. The two concentrations of faeces were 83 9 or 333 g/I artificial saliva. The faeces inoculum was prepared by mixing faeces with CO₂ saturated artificial saliva (McDougall, 1948), straining through two layers of muslin. Fifty ml of inoculum was added to 0.55 9 forage sample in each tube for the in vitro fermentation.

Source of Information: Bang.J. An. Sc.1997, 26(1-2): 31-37 (ISSN 0003-3588)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The dry matter digestibility (DMD) increases with increasing incubation time for both faceal (301, 590 and 638 g/kg for low and 359, 625 and 675 g/kg for high) and rumen liquor (274, 541, and 663 g/kg for low and 396, 604 and 734 g/kg for high). The Organic Matter Digestibility (OMD) increases with increasing incubation time for both faecal (259, 529 and 560 g/kg for low and 348, 579 and 670 g/kg for high) and rumen liquor (260, 506 and 619 g/kg for low. and 368, 583 and 717 g/kg for high). The interaction effect of sources, concentrations and time on OMD and DMD did not show any significant (P>0.05) effect. This experiment suggests further investigation with faecal liquor with extending incubation period to use as source of micro-organisms for in vitro digestibility assay.

Knowledge Management: Journal