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Screening of Salt Tolerant Barley Germplasm Using Nacl

A.S.M.H. Masum
Scientific Officer
Plant Breeding Division, BARI, Gazipur

M.A. Matin
Principal Scientific Officer
Plant Breeding Division, BARI, Gazipur

Abstract/ Executive Summary:

The experiment was conducted in the laboratory of Plant Breeding Davison, BARI, Joydebpur, Gazipur during rabi season 2003-04. 16 germplasm were included in this trial for preliminary screening of salt tolerant germplasm against different concentration of NaCl solutions.

Keywords: Barley, Screening,Saline

Location: Gazipur

Start Date: 00-00-2003

End Date: 00-00-2004

Program Area: Variety and Species

Commodity: Barley

Objectives:

To select salt tolerant barley lines

Methodology:

This experiment was conducted during season of 2003-04 in the laboratory of Pant Breeding Division, BARI, Joydebpur, Gazipur. The trial included 16 germplasm to observe their tolerance against different concentration of NaCl solution. Twenty seeds of each germplasm were taken to test their germination ability against 0.10 % to 1.0 % and 2-10% NaCl solution. Data on percent germination, root length, shoot length; root and shoot ratio and number of root were recorded.

Source of Information: Annual Research report 2003-04, Plant Breeding Division, BARI

Article Link (Online Journal):

Funding Source:

1.	Budget: Tk.40,000/-

Total Budget (Tk.) : Tk.40,000/-

Achievement/Findings:

The entries did not show any response against NaCl of 2 to 10% solution. Different levels of salt tolerance were observed for 0.1 to 1.0% NaCl solution. Mean, range, standard deviation and coefficient of variation for % germination, root length, shoot length, shoot and root length ratio and number of root were calculated and presented in the following tables. Increasing concentration of NaCl solution decreases germination percentage of barley. Germplam showed maximum efficiency in germination for control (70.35%) followed by 0.1% NaCl solution (55.62%) and minimum for 1.0% (20.31%) NaCl solution. BHL-01 (55%) followed by BHL-16 (40%) showed highest level of salt tolerance in terms of percent germination against the high concentration (1.0%) of NaCl solution. Maximum coefficient of variation for germination percentage was observed for 1.0 NaCl solution which gave the scope to select salt tolerates entries.Root and shoot growth at initial stage is very much important for crop establishment, especially for the stress environment. In general, effect of a high salt content in the soil is to give a dwarfed, stunted plant. Root shoot ratio is the indicator of stunted plant. More root shoot ratio indicates the more stunted plants. As the salt concentration becomes higher the stunting becomes noticeable, leaves of the crop become dull-colored and often bluish green and they become coated with a waxy deposit. A significant variation among the lines was observed in the development of root and shoot. Increased NaCl concentration reduced the root and shoot growth for all the genotypes studied. Maximum variation for root and shoot length and also number of root per seedling was observed in the concentration of 1.0% (130.83 %, 137.53% and 111.10% respectively). Hence there is also scope for selection of desired germplasm.

Knowledge Management: Report/Proceedings