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Effects of Media on Callus Induction and Plant Regeneration

Md. Shamsher Ali
Chief Scientific Officer and Head
Biotechnology Division Bangladesh Rice Research Institute Gazipur-1701

Md. Enamul Hoque
Senior Scientific Officer
Biotechnology Division Bangladesh Rice Research Institute Gazipur-1701

AKM Ruhul Baksha
Senior Scientific Officer
Biotechnology Division Bangladesh Rice Research Institute Gazipur-1701

Jannatul Ferdous
Scientific Officer
Biotechnology Division Bangladesh Rice Research Institute Gazipur-1701

Abstract/ Executive Summary:

Anther culture is a technique used in complement to conventional breeding for development of varieties with the desired traits in a shorter period of time by producing double haploid plants. Eveb potential lines fixed through anther culture. Therefore, anthers of promising F₁ s are cultured in the hope of generating potential lines. Anthers of twenty materials (15 F₁_s, and 5 varieties) were planted in KA, KC, KE and M10 media. Twelve F₁s and 5 varieties responded to callus induction. Highest callus induction (16.33%) was obtained from hybrid anthers of BR6157-R1-1X BR4909-R1-R2 followed by BR827-35-2-1 X Khazar (10.88%) in KA medium. Medium number of green plants (249) were regenerated from BR827-35-2-1 X Khazar in KA medium followed by Niamat X Sadiq in M10 medium

Keywords: Anther culture, KA, KC, KE and M10 induction medium

Location: Biotechnology Lab, BRRI, Gazipur-1701

Start Date: 01-07-2005

End Date: 30-06-2006

Program Area: Variety and Species

Commodity: Rice

Objectives:

To develop premium quality rice through anther culture

Methodology:

Twenty F₁s populations were grown in the net house under optimum management. Boots with appropriate stage (uninucliate/mid uninucleate at reduction division stages) were collected from F₁ plants. First the boots were pre-incubated in a refrigerator at 8ºC for 8 days. The panicles were then taken from the boots and cut into small pieces. They were then surface sterilized by immersing 70% ethanol for 2-3 minutes and then placed onto the sterile filter paper in Petri dishes to remove excess alcohol. For callus induction, anthers were incubated into four different media (KA, KC, KE and M10) and kept in incubator under dark condition at 25ºC until cullus initiation. Calli were then transferred into regeneration medium (MS medium containing 1.0 mg/l NAA+ 1.0 mg/l Kn), and culture were then incubated in a culture room under cool white Fluorescent lamps (approximately 1000 lux, under 25ºC) for regeneration. Eight weeks after transfer of calli into the regeneration medium, data were taken on number of anther plated, number of callus produced, number of green and albino plant regenerated.

Source of Information: Proceedings of the BRRI Annual Internal Review for 2005-2006

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Induction KA and M10 media was resposible for good regeneration

Knowledge Management: Report/Proceedings