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Screening for Salinity Tolerance of BRRI Gene Bank Germplasms at the Seedling Stage

Salma Akter
Scientific Officer
Plant Physiology Division BRRI, Gazipur-1701

Rumena Yasmeen
Principal Scienticfic Officer
CSO and head (Additional charge) Plant Physiology Division BRRI, Gazipur-1701

Md. Sazzadur Rahman
Senior Scientific Officer
Plant Physiology Division BRRI, Gazipur-1701

Md. Khalequzzaman
Chief Scientific Officer
CSO and head GRS Division BRRI, Gazipur-1701

Abstract/ Executive Summary:

The experiment was conducted to screen out salt tolerant germplasm at seedling stage. Eighty one germplasm with one BRRI variety were screened at high saline condition using hydroponic system. Among them, only 14 genotypes as Acc no. - 88, 92, 93, 96, 99, 116, 120, 123, 132, 169, 170, 173, 174 and 195 showed visual score 5 that is moderately tolerant. However, other genotypes shows visual score 7 to 9 that is susceptible to highly susceptible at high saline condition.

Keywords: Germplasm, Salinity, Seedling stage

Location: Net house, Plant Physiology Division

Start Date: 15-07-2012

End Date: 08-08-2012

Program Area: Crop-Soil-Water Management

Commodity: Rice

Objectives:

To identify salinity tolerant germplasm at seedling stage.

Methodology:

Eighty one BRRI Gene Bank accessions and 1 BRRI variety (BRRI dhan49) along with tolerant and susceptible check were considered for this experiment. Sprouted seeds were sown on Nylon net fitted with Styro-foam floating on Yoshida full strength culture solution according to Gregorio et al., 1997. Salinity stress @ 12 dS/m was applied 7 days after sowing. The culture solution was changed once a week throughout the experimental period. The pH and EC of the culture solution was checked daily and maintained at 5.0 and 12 dS/m respectively. In each tray FL478 and IR29 were used as a tolerant and susceptible check, respectively. When susceptible check IR29 died, visual score was made according to SES system and living plants were recorded. The experiment was conducted in RCB design with 2 replications.

Source of Information: Proceedings of BRRI Annual Research Review Workshop for 2012-2013, BRRI, Gazipur-1701.

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Forteen (14) germplasm were identified as moderately tolerant to salt stress at the seedling stage. These germplasm could be used for future breeding program to develop new tolerant variety.

Knowledge Management: Report/Proceedings