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Mapping of Ufra Resistant Gene (2009-10)

Dr. Md.Abdul Latif
PSO
Biotechnology Division, BRRI, Gazipur

Md.Monirul Islam
SSO
Biotechnology Division, BRRI, Gazipur

Dr,Md.Enamul Hoque
CSO and Head (In Charge )
Biotechnology Division, BRRI, Gazipur

Dr. Md. Shamsher Ali
CSO
Biotechnology Division, BRRI, Gazipur

Abstract/ Executive Summary:

Disease is one of the most important factors which hinders the yield of rice plant. Among the major diseases ufra affects the rice field in all the three rice growing season and yield loss due to this disease may be 100 percent under favorable environment condition. Chemical control which is effective against this disease to some extent costly and also not environmentally sound. Resistant variety is the most sustainable technology to combat diseases. Gene mapping can identify the exact position and linkage groups of the genes for resistant to ufra diseases. Information about the positions of the resistant genes and the molecular markers linked to them will be useful for marker-assisted selection in ufra resistant rice breeding. A total of one hundred and three primers were surveyed and only thirteen were found polymorphic, which could be used for varieties development.

Keywords: Diseases, DNA extraction, QTL analysis

Location: Biotechnology Division, BRRI-Gazipur

Start Date: 01-01-2009

End Date:

Program Area: Variety and Species

Commodity: Rice

Objectives:

To locate the resistant QTL in a chromosome of ufra resistant variety

Methodology:

One hundred individuals (50 highly susceptible and 50 highly resistant) of F₂ population selected from cross, IR71362-3B-8-3-1-2B/R16-06-3. IR71362-3B-8-3-1-2B is a HYV susceptible while R16-06-3 is an ufra resistant line. Leaf samples from selected individuals along with parent were collected at 30 DAS for DNA extraction and those lines were transplanted in the field. DNA extraction were done following modified CTAB method. Phenotypic study were done in same F₂ generation following inoculation method of 100 nematodes/ plant. For genotypic study, collected DNA was amplified using SSR primers and run electrophoresis. The banding pattern was visualized by staining in ethidium bromide and documented. Linkage analysis was performed by using the Map Manager/QTX computer program (Manly et al. 2001).

Source of Information: Proceedings of the BRRI Annual Internal Review for 2009-10

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

One hundred and three primers were surveyed and thirteen were found polymorphic.

Knowledge Management: Report/Proceedings