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Effects of Dietary Vitamin E on the Growth and Breeding Performance of Ompok Pabda

M.N. Sarowar
Department or Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M.F.A. Mollah
Department or Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

A 90 day feeding trail was conducted to investigate the effect of vitamin E on the growth and breeding performance of Ompok pabda. A total of 84 healthy female broodfish (41.10±0.44g) were divided into 4 treatments i.e. treatment T₁, T₂, T₃, and T₄, having three replications each. The fish were fed twice a day with a standard feed (40% protein) having 4 doses of vitamin E viz. 0 (served as control), 50, 100 and l50mg vitamin E/kg feed. At the end of the feeding trial, the broodfish were induced to breed with PG extract to observe the effect of vitamin E on feed. After rearing for 90 days with the experimental feeds, it was found that weight gain and specific growth rate of broodfish fed with 100mg vitamin E/kg feed (treatment T₃) was the highest (14.78±0.38g and 2.99±0.11) while l50mg vitamin E/kg feed (treatment T₄) fed fish gave the poorest result (2.97±0.89g and 1.21 ±0.32). There was no significant difference in terms of length gain of broodfish among the different treatments. The brood fish were induced to breed with equal dose of PG extract (18 and l2mg PG/kg body weight for female and male respectively) to observe the dietary effect of vitamin E on breeding performance. The highest ovulation, fertilization and hatching rate of eggs were found to be 81.48±6.4l %, 84.04±3.53% and 68.59±5.03% respectively in the broodfish of treatment T, while the poorest (33.33±00%, 52.35±5.02% and 45.70±7.24% respectively) were found in the broodfish under treatment T₄. The results suggest that inclusion of 100mg vitamin E/kg feed is best for enhancing the breeding performance of O.pabka broodfish indicating that vitamin E content has a positive impact on reproduction of fish. The present results also imply that inclusion of higher level of vitamin E exerts an antagonistic effect in terms of growth and breeding performance of this species.

Keywords: Growth rate, Breeding, Vitamin E, Onipok pabda

Location: Faculty of Fisheries, Bangladesh Agricultural University (BAU), Mymensingh

Start Date: 01-04-2009

End Date: 30-06-2009

Program Area: Variety and Species

Commodity: Fish

Objectives:

To observe the effect of vitamin E on growth and breeding performance of O. pabda broodfish.

Methodology:

The research work was conducted in 12 cisterns (l20x70x40cm³) of the mini hatchery cum breeding complex, Faculty of Fisheries, Bangladesh Agricultural University (BAU), Mymensingh. The breeding trials on the other hand, were carried out in the hapas of 150xl00xI100cm³ affixed in the pond beside the hatchery complex with the help of bamboo poles. Prior to stocking of brood fish, each of the cisterns was installed with all the facilities necessary to run the experiment efficiently. An inlet and an outlet were provided with each of the cisterns to facilitate renewal and removal of water concomitantly. Water hyacinths were kept floating at a corner of each cistern with the help of bamboo frame attached with float to provide shelter to the experimental fish. Water hyacinth was used to keep the water cool and clean. Adequate number of mature and healthy O. pabda were collected from the wild source during the month of February and March 2009 and acclimatized in the cisterns. A total of 84 healthy, strong, and similar sized females were selected for rearing as broodfish for the research work. The brood rearing experiment was started on I April 2009 and continued up to 30 June 2009. There were four treatments (i.e. T_1, T_2, T₃ and T₄,) each with 3 replications stocked with 7 females. The broodfish of treatment T, served as control (i.e. fed vitamin E free diet) while that of treatment T₂' T₃ and T₄ were fed with a feed having 50,100 and 150 mg vitamin E/kg feed respectively.

The experimental feeds were administered directly into the corresponding cisterns twice daily ad libitum. Each of the cisterns was siphoned every morning to remove faeces and uneaten feed particles. During sampling at 15 days interval, all the fish from each replication of a treatment were caught and weight (g) and length (cm) of each fish were measured by using a sensitive electric balance and a measuring scale respectively and recorded. During the experimental period temperature, dissolved oxygen and pH were recorded weekly.

For breeding trial, a total of 36 broodfish from [our treatments were selected and kept in 4 separate cisterns for about 6 hours under gentle but continuous shower for conditioning prior to injection with PG extract at a dose of 12 and 18mg/kg body weight of male and female respectively. Mature and healthy males of O. pabda collected from the broodstock pond were kept in fiberglass tank. After injecting PG extract, both females and males were kept together treatment wise in the hapa set in a pond for spawning. Most of the broodfish were found to oviposit within 9 to 10h post injection. The broods were removed from the hapas after 10.5h of injection when the spawning was completed.

For calculation of fertilization and hatching rates of eggs produced by the females of each treatment, a portion of the eggs were taken and incubated in 12 bowls of 40cm diameter corresponding to the treatments, i.e. three replications for each treatment. The remaining eggs were incubated in separate trays. Soon after fertilization, the embryonic development started and the fertilized eggs looked watery and slightly transparent. Within 6 hours of incubation, the numbers of fertilized and unfertilized eggs from each bowl for respective treatment were counted based on the appearance of the eggs. The unfertilized eggs turned opaque and whitish in colour few hours post fertilization. After completion of hatching, the number of larvae of each bowl was counted by siphoning them out. The hatching completed within 22h at 24-25°C

Source of Information: Bangladesh Journal. Fish. Res., 13(2),2009: 105-114

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The results suggest that inclusion of 100 mg vitamin E/kg feed is best for enhancing the breeding performance of O.pabka broodfish indicating that vitamin E content has a positive impact on reproduction of fish. The present results also imply that inclusion of higher level of vitamin E exerts an antagonistic effect in terms of growth and breeding performance of this species.

Knowledge Management: Journal