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Biochemical Attributes of Mutant Rice Under Different Saline Levels

Mohammad Zahidul Islam
Scientific Officer
Genetic Resources and Seed Division, BRRI Gazipur-1701

M A Baset Mia
Professor
Department of Crop Botany BSMRAU,Gazipur-1706

Anowara Akter
Scientific Officer
Hybrid Rice Division, BRRI Gazipur-1701

Md. Hafijar Rahman
Scientific Officer
Hybrid Rice Division, BRRI Gazipur-1701

Abstract/ Executive Summary:

A pot experiment was conducted at the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh to find out the biochemical attributes of mutant rice under varied saline levels during T-aman season from July to November 2003. Three rice genotypes viz. Q-31, Y-1281, and MR-219 were used as tests materials. The experiment was laid out in a Randomized Complete Block Design (RCBD) with four replications. Six salinity levels namely 15 dSm^-1, 12 dSm^-1, 9 dSm^-1, 6 dSm^-1, 3 dSm^-1 with a control were imposed in this experiment. Results of the experiments clearly showed that biochemical characters such as total chlorophyll content, nitrate reductase activity, K+ and Ca++ etc. content of straw were reduced with increase levels of salinity compared to control. On the other hand, Na+ were increased with increasing salinity levels. Among the genotypes, MR-219 showed best performance in respect of salt tolerant up to 6 dSm^-1 and showed Y-1281 intermediate status. The genotypes Q-31 and Y-1281 showed its susceptibility to salinity stress.

Keywords: Salinity stress, Biochemical attributes and Mutant rice

Location: BINA, Mymenshing

Start Date: 01-01-2003

End Date: 12-12-2003

Program Area: Variety and Species

Commodity: Rice

Objectives:

The present research work was undertaken to estimate the biochemical attributes (total chlorophyll content, nitrate reductase activity, Na+, K+ and Ca+) of mutant rice under different saline levels.

Methodology:

A pot culture experiment was carried out in Bangladesh Institute of Nuclear Agriculture (BINA) of which each pot contained 8.0 kg of dried soil, belongs to the Sonatola series of Grey Flood Plain under the Old Brahmaputra Agro-Ecological Zone (UNDP and FAO, 1988). The pH value, cation exchange capacity (CEC) and electrical conductivity (EC) of the soil were 6.44, 6.78meq/100 g soil and 0.6 dSm^-1 respectively. Three rice genotypes Q- 31, Y-1281 and MR-219 were used in the present in this experiment. Earthen pots of 24.5 cm top diameter, 14 cm bottom and 30 cm depth were used, which pot was filled with 8 kg sun-dried soil. A polythene lining was provided inside the pots. N, P, K and S applied at rate of 60-8-30-4 kg/ha in the form of Urea, TSP, MP and Gypsum according to Fertilizer Recommendation Guide (BARC, 1997). The following salinity treatments namely control, 3, 6, 9, 12 and 15 dSm^-1 were imposed in the experiment. Salinity treatments were given at 33 days after transplanting (DAT). EC was measured by the electrical conductivity bridge at room temperature and calculated to the standard temperature of 25⁰ C. The pH and CEC soil were analysis following the methods of (Black, 1965). After collecting the leaf of plants, 0.05 g leaf was weighed and chlorophyll content was determined using the method of Coombs et al. (1985).Nitrate reductase activity was determined from the leaf samples following the method of Steward and Orebamjo (1979).The Na+, K+ and Ca++ contents of straw of the harvested plants were determined by atomic absorption spectrophotometers (model- PERKIN- ELMER, 2380) directly and express in percentages (Jackson, 1967).The collected data were statistically analyzed by MSTAT-C package program develop by (Russel, 1986).

Source of Information: Int. J. Sustain, crop Prod,2(3):17-21 (August 2007)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Results of the experiments clearly indicated that biochemical characters namely chlorophyll content, nitrate reductase enzyme activity, K+ and Ca²⁺ content decreased with the increase of salinity and Na+ increased with the increment of salinity.

Knowledge Management: Journal