Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Regeneration Potentiality of Brassica Genotypes in Differential Growth Regulators

M. M. A. KHAN
Department of Biotechnology, Bangladesh Agricultural University (BAU),Mymensingh

A. B. M. ARIF HASAN KHAN ROBIN
Department of Genetics and Plant Breeding, Bangladesh Agricultural University (BAU), Mymensingh

M. A. N. NAZIM-UD-DOWLA
Plant Genetic Resource Centre, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur

S. K. TALUKDER
Department of Genetics and Plant Breeding, Bangladesh Agricultural University (BAU),

L. HASSAN
2Department of Genetics and Plant Breeding, Bangladesh Agricultural University (BAU), Mymensingh

Abstract/ Executive Summary:

Petiole of six genotypes of oilseed Brassica viz. Tori-7, Sampad, Kallyania, BARI Sarisha-7, BARI Sarisha-8, and MM 20-3 were cultured in MS medium with different concentrations of BAP, NAA, and AgNO3 for callus induction and subsequent plant regeneration. The highest percentage of callus induction (91.43%) was observed in Tori-7 in the media supplemented with 2 mg/L BAP, 0.1 mg/L NAA and 2.0 mg/L AgNO₃. Calli were maintained in order to get sufficient number of regenerants. With the increased concentration of BAP, the highest percentage (57.14) of regenerants were found in Tori-7 followed by Sampad (33.13%) and BARI Sarisha-8 (31.42%) in MS media supplemented with 2.5 mg/L BAP, 0.1 mg/L NAA and 2.0 mg/L AgNO₃. Root formation from the regenerants was found best in half MS medium supplemented with 0.5 mg/L NAA in genotype Tori-7. Regenerated plantlets of four genotypes (Tori-7, BARI Sarisha-8, Kallyania, BARI Sarisha-7) were successfully established in the field.

Keywords: AgNO3, BAP, Brassica, NAA, regeneration

Location: BAU, Mymensingh

Start Date: 01-09-2004

End Date: 31-07-2005

Program Area: Variety and Species

Commodity: Mustard

Objectives:

present study was undertaken to establish a suitable and reproducible protocol for in vitro regeneration of Brassica rapa and Brassica napus varieties.

Methodology:

The experiment was conducted at the tissue culture laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh during the period from September 2004 to July 2005. Six oilseed Brassica genotypes were used for this experiment. The genotypes were Tori-7, Sampad, Kallyania of Brassica rapa and BARI Sarisha-7, BARI Sarisha-8, and MM 20-3 of Brassica napus. Seeds of Sampad were collected from Bangladesh Agricultural University (BAU) and those of other five genotypes were collected from Bangladesh Agricultural Research Institute (BARI), Gazipur. Half strength MS (Murashige and Skoog, 1962) medium supplemented with 20 mg/L sucrose was used for seed germination. For callus induction and shoot regeneration, five different combinations each containing MS + 0.l mg/L NAA+ 2 mg/L AgNO₃ with different concentrations of BAP viz., 0 ppm, 1 ppm,1.5 ppm, 2 ppm, and 2.5 ppm were used. Again, for root initiation, three treatments each containing half-strength MS medium supplemented with three different concentrations of IBA (0 ppm, 0.1 ppm, and 0.5 ppm) were used. The regenerated plantlets were transplanted from culture vessel to plastic pots containing 25% garden soil + 50% Sand + 25% Cowdung in growth chamber. For callus induction, data were recorded on days to callus initiation, percent callus induction and nature of callus (loose, friable, and compact texture of callus were scored one, two, and three, respectively); for plantlet regeneration data were recorded on days to shoot initiation, number of callus showing shoots, total number of shoots per petridish and for root initiation, data were recorded on percent shoot showing root. The collected data were analyzed with proper statistical methods.

Source of Information: Bangladesh J. Agril. Res. 35(2) : 189-199, June 2010 ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Mean values of six genotypes were found statistically significant for all the characters of shoot regeneration like number of calli with shoots/petridish and number of shoots/callus. Number of calli with shoot was highest in Tori-7 and lowest in MM 20-3. It was observed that among the genotypes tested, Tori-7 showed maximum number of shoots/callus followed by Sampad, BARI Sarisha-8, and Kallyania Results related to hormone × genotype interaction for the characters of shoot regeneration, such as number of callus with shoot per petridish and number of shoots per callus. These parameters were found statistically significant, indicating significant differences among the interactions for those characters. Number of callus with shoots per petridish was highest on T₅×Tori-7 (4.0) and T₄ × Tori-7 (3.8) whereas, lowest was found in T₁ with BARI Sarisha-7(0.20). Number of shoots/callus was highest (3.5) on T₅× Tori-7 and lowest (0.40) in T₁ × BARI Sarisha-7. From the above discussion, it may be concluded that T₄ performed best with the interaction of Tori-7 for shoot regeneration Induction of root from regenerated shoots showed wide variations according to genotypes and different concentrations of NAA in the medium. Tori-7 had the highest percentage (55.56) of rooted shoots irrespective different concentrations of NAA. Mean values due to different concentrations of NAA for number of shoots with root were highly significant, indicating the presence of variation among the concentrations used for this study. It indicates that irrespective of genotypes, maximum number of shoots with root (44.44%) was found in 1/2 MS + 0.5 mg/L NAA followed by 1/2 MS + I mg/L NAA (30%), and 1/2 MS (22.16%). Different genotypes showed significant variation in producing root. Tori-7 showed highest number of shoots with root followed by BARI Sarisha-8, Kallyania) and BARI Sarisha-7. Establishment of plantlets: The regenerated plantlets were transplanted into plastic pots containing sterile soil, sand, and cowdung in a 1: 2: 1 ratio for acclimatization. Gradually the plantlets were adapted to the soil

Knowledge Management: Journal