Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Inheritance of Fertility Restoration Involving Wild Abortive Cytoplasmic Male Sterility System in Rice

Dr. Md. Jamil Hasan
Principal Scientific Officer
Head Hybrid Rice Division Bangladesh Rice Research Institute Gazipur-1701

Priya Lal Biswas
Senior Scientific Officer
Hybrid Rice Division Bangladesh Rice Research Institute Gazipur-1701

Abstract/ Executive Summary:

Inheritance of fertility restoration was studied in crosses involving ten elite restorer lines of rice viz. BR6839-41-5-1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R and one male sterile line Jin23A with WA sources of cytoplasmic male sterility. The segregation pattern for pollen fertility of F₂ and BC₁ populations of crosses involving Jin23A indicated the presence of two independent dominant fertility restoring genes. The mode of action of the two genes varied in different crosses revealing three types of interaction, i.e. epistasis with dominant gene action, epistasis with recessive gene action, and epistasis with incomplete dominance.

Keywords: Cytoplasmic male sterility, Fertility restoration, Inheritance, Oryza sativa.

Location: BRRI HQ, Gazipur

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Rice

Objectives:

To determine the genetic control of fertility restoration of WA-CMS system in rice.

Methodology:

The material consisted of one male sterile line Jin23A belonging to WA type source of cytoplasmic male sterility and ten prospective restorer lines. The restorer lines were BR6839-41-5-1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R. First season F₁ was raised. The resulting F₁’s were selfed as well as backcrossed with their respective female parents to generate F₂ and BC₁ populations in the second season and final season evaluation was made with pollen and spikelet fertility of each plant in the F₂ and BC₁ populations. From each plant, five anthers from different spikelets were collected and their pollen grains were stained in 1% Iodine Potassium Iodide (IKI) solution. Plants were classified on the basis of pollen fertility analysis as fertile (61-100% pollen stained), partially fertile (31-60%), partially sterile (1-30%) and sterile (<1%) based on their shape and extend of staining under an optical microscope. One panicle from each plant was bagged before flowering for spikelet fertility analysis and spikelet fertility of bagged panicle was count at maturity stage. At maturity, the bagged panicle was examined for seed set and classified as fertile (81-100% seed set), partially fertile (31-80%), partially sterile (1-30%) and sterile (<1%). The parental lines, F₁ progenies, 250 F₂ plants for each segregating population were grown and evaluated in the same conditions for phenotypic and pollen fertility rate was used as the main criteria for the evaluation of fertile and sterile plants. F₁’s contained two rows, F₂ seven rows, BC₁ five rows with 37 plants/row and non replicated. For inheritance analysis plants with less than 1% stained pollen were categorized into the sterile class, and all others were regarded as fertile. Chi-square analysis was used to estimate the distribution pattern of Rf alleles with WA type source of CMS lines.

Source of Information: Bangladesh Journal of Plant Breeding & Genetics, 24(1):33-40, 2011

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 40000/-

Total Budget (Tk.) : 40000/-

Achievement/Findings:

Based on the results obtained, it was concluded that the inheritance of male sterility and fertility restoration was conditioned by a pair of alleles, dominant and recessive, at a single locus interacting with the male sterile cytoplasm, and that the mode of action of the two genes varied in different crosses revealing three types of interaction, i.e. epistasis with dominant gene action, epistasis with recessive gene action, and epistasis with incomplete dominance.

Knowledge Management: Journal