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Bacterial Leaf Blight of Wheat

A. Q. M. Bazlur Rashid
Bangladesh Institute of Nuclear Agriculture P. O. Box No.4. Mymensingh Bangladesh

M. Jalaluddin
Bangladesh Institute of Nuclear Agriculture P. O. Box No.4. Mymensingh Bangladesh

A. K. Podder
Bangladesh Institute of Nuclear Agriculture P. O. Box No.4. Mymensingh Bangladesh

M. Afsaruddln
Bangladesh Institute of Nuclear Agriculture P. O. Box No.4. Mymensingh Bangladesh

Abstract/ Executive Summary:

Leaf blight disease on wheat was investigated over a period of three consecutive growing years (1980-81 to 1982-83) in a number of fields observed in five different districts of Bangladesh. Symptoms of the disease were described and Pseudomonas syringae van Hall was diagnosed as the causal bacterium. Reactions of some promising wheat cuItivars/ lines to natural infection with the bacterial leaf blight were studied. Girua. .Jupatic, Nabra, Penjamo and w-252 were found to be resistant.

Keywords: Leaf blight, Wheat, Resistant genotype.

Location: Bogra, Dhaka, Jamalpur, Pabna and Mymensingh districts of Bangladesh

Start Date: 01-11-1980

End Date: 31-04-1983

Program Area: Pest Management

Commodity: Wheat

Objectives:

1. To study the prevalence, symptoms and cause of the leaf blight as well as to evaluate the field reactions of some wheat cultivars/lines under trial in the Bangladesh Agricultural University (BAU) farm, Mymensingh.

Methodology:

Natural occurrence of the leaf blight on wheat has been observed since 1980 in different areas of Bogra, Dhaka, Jamalpur, Pabna and Mymensingh districts of Bangladesh. In each district 10 to 27 fields were casually observed for the prevalence of the disease. Twenty five promising wheat cultivars/ lines were evaluated for resistance to the bacterial leaf blight under natural field conditions in the BAU farm, Mymensingh. Symptoms of the disease were recorded at different stages of plant growth. The disease severity was recorded and rated. Isolations of tile pathogen were made from the infected wheat leaves collected from different fields surveyed in the five districts. The infected leaves were rinsed several times in tap water and soaked for 2-5 min in 0.5% sodium hypochlorite. Bits of those washed leaves were then smashed with a sterile glass rod in a small amount of sterile distilled water in a sterile test tube. After the bacteria were allowed to diffuse from the bits in the water, streak plates were prepared with a loop on King's medium B. The plates were then observed 3-4 days after streaking. The bacterial colonies that produced fluorescent water-soluble pigments were detected and transferred to fresh King's medium B and maintained at 5⁰C for further studies. Inoculum was prepared from a three-day-old culture of the bacterium by suspending it in sterile distilled water. Inoculations were done on five susceptible and moderately susceptible wheat cultivars /lines and cowpea seedlings by spraying the bacterial suspension (10 cells per rnilililitre ) on plants 2-3 weeks old by means of air brushes. The same was done on check plants with sterilized distilled water. The inoculated plants were kept in moist chambers for three days at 26-27°C and maintained. Symptoms were recorded one week later.

Source of Information: Bangladesh J. Agril. Sci. 13 (1) : 35-41 : 1986

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Results from inoculation experiments on wheat cvs./line, Pavon, Balaka. Inia 66, MST, Norteno 67 and Sonalika. and also on cowpea seedlings demonstrated that the pathogenic bacterium produced small water-soaked tan-gray spots on inoculated leaves 3-4 days after inoculation.

Knowledge Management: Journal